The trouble of antimicrobial drug resistance has presupposed a search for new antimicrobial substances from other exporters including natural sources. Marine micro-organisms are known to produce metabolites to safeguard themselves against pathogens and therefore can be deemed as a potential source of antimicrobial substances. This research intended to evaluate the antimicrobial activity of six hard coral species namely Acropora hemprichii, Acropora austera Seriatopora hystrix, Seriatopora pistillata, Pocillopora verrucosa and Millepora dichotoma against some pathogenic microbes, and the bioactive compounds were extracted using ethyl acetate. The antimicrobial activity of the extracts was estimated using the disc diffusion method. The organic extract from Pocillopora verrucosa was the most effective against all selected microorganisms except Bacillus subtillus ATCC6633 and Aspergillus flavus while the highest effect was showed against Fusarium solani (22mm). Moreover, a partial description of these agents was carried out using the gas-liquid chromatography (GC-Mass). The main ingredient of Pocillopora verrucosa crude extract organic acids, aldehydes, esters, carotene, and their derivatives. That is the first research about the extraction of natural bioactive compounds from Pocillopora verrucosa which located in Al-Hamraween, Red Sea, Egypt.