Colletotrichum gloeosporioides and Rhizopus stolonifer were cultivated in two culture media: potato dextrose agar (PDA) to determine the maximum growth mycelial speed (μ_max) and lag phase (λ) using the Gompertz and Hills mathematical models, also used liquid medium potato dextrose broth (PDB) to obtain production of mycelial biomass and exopolysaccharides (EPS). Colletotrichum gloeosporioides showed the highest maximum growth rate (0.38 day^-1) and the lowest lag phase duration (1.85 h) on PDA medium, Colletotrichum gloeosporioides cultivated on liquid medium presented the highest biomass production (76.15%) and exopolysaccharides production (80.00%). Exopolysaccharides (EPS) obtained from the liquid culture medium of Colletotrichum gloeosporioides presented the highest absorption content of lead (0.015 mg.g^-1). The results obtained that exopolysaccharides of Colletotrichum gloeosporioides cultivated on PDB medium showed the highest absorption of lead (Pb) in the sediment of the aquaculture pool.