In the order Perciformes, there are many of fishes which have economic importance and have cytogenetic biodiversity. Cytogenetics is becoming an important biodiversity-detection tool and used to measurement biodiversity evolutionary aspects. A remarkable degree of chromosomal conservatism (2n=48, FN=48) has been identified in several families of Perciformes. However, some families exhibit greater karyotypic diversity. The present study was aimed to characterize cytogenetically the chromosomal formula, chromosome numbers and karyotypes of three fish species of order Perciformes; Colisa chuna, Osphronemus goramyand Betta splendens using karyological analysis. All Fish species were collected from ornamental fish farms in Egypt. The diploid chromosome number, chromosomal formula, and fundamental numbers of the three species under study were 2n=46, 20m+26sm and FN=92, 2n=48, 2m+46a and FN=50, and 2n=42, 12sm+14st+16a and FN=68 respectively. These results may open the way for using cytogenetic analysis in modern taxonomy.