This study was conducted on fifty fish samples of Clarias gariepinus randomly collected from Zagazig, Sharkia governorate, Egypt. The collected fish samples were evaluated for heavy metals, antibiotics, and aflatoxin residues. Arsenic and Zinc residues were detected in 100% of fish samples with a mean value of 0.07± 0.007 and 5.13 ± 0.39 ppm respectively, while lead and mercury residues were detected in 90% of fish samples with a mean value of 0.28±0.05 ppm and1.4 ± 0.24 ppm respectively. On the other hand, cadmium residue was detected in 50% of fish samples with a mean value of 0.018 ± 0.002 ppm. Sulfadimidin residue was detected in all examined Clarias gariepinus samples with a mean value ± SD (50.97±21.55 µg/kg). All examined fish samples were below maximum residue limit 100 μg/ kg while aflatoxin was detected only in one fish sample with a concentration 5.32 µg/kg (ppb) which declared that there is no human health risk from consumption of Calais gariepinus flesh.