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96005

Establishment of a cell culture system and characterization of the primary cultures from different organs of the Nile tilapia Oreochromis niloticus

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Last updated: 01 Jan 2025

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Abstract

In addition to toxicology, carcinogenesis, functional genomics studies, and lacking in vivo complexities, fish cell culture considered necessary to study virus-host interactions and isolate viral pathogens in fishes. Therefore, the present study aimed to establish a cell culture system and characterize the primary culture from different organs of Nile tilapia Oreochromis niloticus fish. Leibovitz-15 (L-15) medium containing L. glutamine and supplemented by 15% of Foetal Bovine Serum (FBS), 200 IU/ml penicillin, 200 µg/ml streptomycin and 2.5 µg/ml amphotericin were used for culturing the cells. In this study, Primary cultures from different organs (brain, testis, and ovaries) from Oreochromis niloticus were established. The results of the present investigations showed that brain primary cultures grew slowly. Active growth was observed in ovary primary culture on the 7th day with fibroblast-like growth. Testis primary culture showed active growth on the 2nd day and was capable of maintaining organ function. 

DOI

10.21608/ejabf.2020.96005

Keywords

Oreochromis niloticus, primary cell culture, Brain, testis, Ovary

Authors

First Name

Medhat

Last Name

H. Hashem

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Orcid

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First Name

Dina

Last Name

T. Meshhal

MiddleName

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Orcid

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First Name

Fatma

Last Name

A. S. Mohamed

MiddleName

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Affiliation

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Orcid

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First Name

Omaima

Last Name

A. Khamiss

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Orcid

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Volume

24

Article Issue

4

Related Issue

14113

Issue Date

2020-07-01

Receive Date

2020-04-30

Publish Date

2020-07-01

Page Start

127

Page End

137

Print ISSN

1110-6131

Online ISSN

2536-9814

Article File

EJABF_Volume 24_Issue 4_Pages 127-137.pdf

PDF . 547.9kB

Link

https://ejabf.journals.ekb.eg/article_96005.html

Detail API

https://ejabf.journals.ekb.eg/service?article_code=96005

Order

8

Type

Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Establishment of a cell culture system and characterization of the primary cultures from different organs of the Nile tilapia Oreochromis niloticus

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Article

Created At

22 Jan 2023