93528

Immunogenization of Heat-Killed Vaccine Candidate from Aeromonas hydrophila in Catfish (Pangasius hypophthalamus) using Strain of Banjar, South Kalimantan, Indonesia

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Last updated: 01 Jan 2025

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Abstract

Aeromonas hydrophila often attacks cultured catfish and causes motile aeromonad septicemia (MAS) disease outbreak in South Kalimantan, Indonesia. Deaths from A.hydrophila attacks could reach 100% so that prevention needs to done through vaccination. This study aimed to examine the potential immunogenicity of 6 heat-killed A.hydrophila vaccine candidates, a strain of Banjar, South Kalimantan, Indonesia. A.hydrophila strains obtained from infected catfish in aquaculture ponds around the Banjar District, South Kalimantan, Indonesia. From 10 fish infected with MAS, obtained 14 isolates of bacteria, ie, 8 isolates (AGC-1, AGC-2, AGC-3, AGC-4, AGC-6, AKC-2, AKC-3, and AKC-5) of Sungai Batang village, and 6 isolates (AGC-8, AGC-9, AKC-7, AKC-8, AKC-9, AKC-10) from Cindai Alus village. AGC signifies Aeromonas isolated from the gills, and AKC means Aeromonas isolated from the kidney. The antigen that used as a candidate for the heat-killed A.hydrophila vaccine made by inactivation through a heating process at 100 oC for 60 minutes. The results showed antigens from AGC-2 and AGC-8 strains had high immunogenicity because they could increase antibody titers compared to other strains and controls.  The antibody titer in catfish, two weeks after booster vaccination, increased and showed the same value. The results of the cross-reaction assay showed that the antigens from the AGC-2 and AGC-8 strains were able to cross-react with strain AGC-1, AKC3, AKC-5, but unable to cross-react with AKC-7, so that AGC-2 and AGC-8 could be recommended as vaccine candidates for MAS disease in South Kalimantan, Indonesia.

DOI

10.21608/ejabf.2020.93528

Keywords

Aeromonas hydrophila, Antibody, Immunogenicity, Pangasius hypophthalamus, vaccine

Authors

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Olga

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Olga

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olgafikan@gmail.com

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Orcid

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First Name

Siti

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Aisiah

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sitiaisiah@ulm.ac.id

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Wendy

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A. Tanod

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Yenny

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Risjani

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risjani@ub.ac.id

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First Name

Happy

Last Name

Nursyam

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happy_nsy@ub.ac.id

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Orcid

0000-0002-6508-5493

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Maftuch

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Maftuch

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maftuch@ub.ac.id

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Volume

24

Article Issue

4

Related Issue

14113

Issue Date

2020-07-01

Receive Date

2020-02-13

Publish Date

2020-07-01

Page Start

1

Page End

13

Print ISSN

1110-6131

Online ISSN

2536-9814

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https://ejabf.journals.ekb.eg/article_93528.html

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https://ejabf.journals.ekb.eg/service?article_code=93528

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Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Immunogenization of Heat-Killed Vaccine Candidate from Aeromonas hydrophila in Catfish (Pangasius hypophthalamus) using Strain of Banjar, South Kalimantan, Indonesia

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Article

Created At

22 Jan 2023