Peacock bass (Perciformes, Cichlidae, Cichla)are multi-coloured and highly predatory fish originated from the Amazonian region. The species was deliberately introduced into Malaysia freshwater bodies by anglers in the early 1990s for sport fisheries. In this recent study, we found the population of peacock bass in Lake Telabak, a man-made lake in Besut, Terengganu. Using the mitochondrial DNA analysis approach, the origin and taxonomy of peacock bass in the lake were clarified. A total of forty fishes were sampled from Lake Telabak for the analysis. Haplotype was detected among all samples. The current study revealed that Cichla spp. in Lake Telabak are closer to Cichla ocellaris (Bloch and Schneider, 1801) with the highest sequence similarity of 99.84% as blasted at the National Center for Biotechnology Information (NCBI) database. The evolutionary history inferred using the maximum likelihood method in the molecular phylogenetic analysis also confirmed the species as C. ocellaris. The origin of Cichla spp. in the current study was traced to the Amazonas and Parà states of Brazil (Manaus, Novo Airão, Tapajós, and Tefé).