This study provides knowledge on otolith morphometrics and the relationships between otolith dimensional length, weight, body height, and a head length of Glossogobius sparsipapillus. A total of 572 individuals were measured in this study, of which 308 males and 264 females. The samples were collected from April 2019 to January 2020 at three studied sites from Vinh Hau, Hoa Binh, Bac Lieu to Dien Hai, Dong Hai, Bac Lieu and Tan Thuan, Dam Doi, Ca Mau. The length, width, and weight of the otolith did not differ between the left and right of otoliths as well as males and females. However, the differences in length, width, and weight of otoliths varied among the three studied sites. The weight of the otolith was closely related to the length, weight, body height, and head length of the fish. The results showed that otolith weight can be used to determine fish size.