The aim of this work was to investigate the effect of chitocanitosan nanoparticles (CsNPs) biosynthesis using Spirulina platensis as supplemented food in black tiger shrimp (Penaeus monodon) diets. Therefore 6 diets were formulated to provide 45% crude protein and 499k cal./100 g diet and supplemented using S. platensis and chitosan as follow: T1: control, T2: 0.05% S. platensis, T3: 0.1% S. platensis, T4: 1 ml chitosan nanoparticles, T5: 1 ml chitosan nanoparticles with 0.05% S. platensis and T6: 1 ml chitosan with 0.1% S. platensis. Each diet was offered at 5% feeding rate twice, 6 days/ week in duplicate shrimp groups (1.24 g/fingerling) stocked at 15 fingerlings per m³ for each of 12 (1×2.5×1m³) hapas for 12 weeks. Growth performance, some hematological parameters and blood components of P. monodon were determined after 84 days. Supplementation of the basal diet using each of spirulina and chitosan nanoparticles (T5 and T6) significantly improved the final body weight (BW), body length (BL) and Daily weight gain (DWG) and P. monodon fed the diet T5 (supplemented using 1ml chitosan and 0.05% S. platensis) and T6 (supplemented using 1ml chitosan and 0.01% S. platensis) showed the highest serum protein, cholesterol and lactate of black tiger shrimp (P. monodon). Accordingly, it could be concluded that feeding P. monodon with diets containing spirulina and chitosan nanoparticles diets improves the growth performance, blood biochemical parameters of the P. monodon and therefore improves their production.R).