Beta
ManageSearchChatDiscover
70593

Detection of Nervous Necrosis Virus of Grouper Fish in Lombok Island, Indonesia Based on Serological and Molecular Studies

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Cantang grouper fish (Epinephelus fuscoguttatus x Epinephelus lanceolatus) is one of the marine fisheries commodities that have opportunities both in the domestic and international markets. The cultivation of marine fish in Indonesian water has been largely harmed by the outbreak of viral diseases, for example, those caused by Nervous Necrosis Virus (NNV). The aim of study is to: produce primary antibody of NNV; and to detect disease based on immunohistochemical (IHC) and molecular methods. Two New Zealand rabbits (Oryctolagus cuniculus) of 8 months old males, ± 2 kgs of weight were vaccinated against NNV disease each week in a dose of 0.50 cc, 1.00 cc, 2.00 cc, and 3.00 cc by intra-peritoneal injection. The rabbit serum was harvested in the fifth week activated, and filtered by membrane dialysis three times to obtain immunoglobulin as the primary antibody. The brain, eyes, and spleen of infected grouper, were processed for histological tissue and stained with Hematoxylin and Eosin and immunohistochemical methods. Positive results were supported by the molecular test of Polymerase Chain Reaction (PCR) with Nishizawa's primers: R3: 5 '-CGA GTC AAC ACG GGT GAA GA-3' and F2: 5 '-CGT GTC AGT CAT GTG TCG CT-3'. The NNV primary antibody was sensitive for the detection. The positive results of IHC stains were supported by the PCR test in grouper fish from Lombok.

DOI

10.21608/ejabf.2020.70593

Keywords

Cantang grouper, E. fuscoguttatus, E. lanceolatus, Lombok Island, NNV, IHC, PCR

Authors

First Name

Arief

Last Name

Sulistiyono

MiddleName

-

Affiliation

-

Email

drh.arief_sulistiyono@yahoo.com

City

-

Orcid

-

First Name

Kurniasih

Last Name

Kurniasih

MiddleName

-

Affiliation

-

Email

kurniasih_1951@yahoo.co.id

City

-

Orcid

-

First Name

Tri

Last Name

Untari

MiddleName

-

Affiliation

-

Email

t_untari@ugm.ac.id

City

-

Orcid

-

Volume

24

Article Issue

1

Related Issue

10207

Issue Date

2020-01-01

Receive Date

2019-10-11

Publish Date

2020-02-01

Page Start

329

Page End

335

Print ISSN

1110-6131

Online ISSN

2536-9814

Article File

EJABF_Volume 24_Issue 1_Pages 329-335.pdf

PDF . 506.1kB

Link

https://ejabf.journals.ekb.eg/article_70593.html

Detail API

https://ejabf.journals.ekb.eg/service?article_code=70593

Order

19

Type

Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Detection of Nervous Necrosis Virus of Grouper Fish in Lombok Island, Indonesia Based on Serological and Molecular Studies

Details

Type

Article

Created At

22 Jan 2023