An experiment was conducted to compare the effect of three levels of nanoparticles iron (nFe₂O₃) and zinc (nZnO) with the same levels of bulk Fe (Fe₂O₃) and Zinc (ZnO) as feed additive on growth performance, feed efficiency, chemical body composition and tissue accumulations of Fe and Zn of Nile tilapia fingerlings (17.73 g). A basal diet (30.71%CP) was used with all treatments. This experiment consisted of seven. The fish reared in fiberglass tanks (1.5m3). The fish fed twice daily 8: am and 4: pm, a rate of 5% of the biomass. The experimental period was 80 days. Statistical analysis showed significant differences between the treatments in growth parameters, feed efficiency parameters, body chemical composition, and tissue accumulation of Fe and Zn. T6 (40 mg Nano iron oxide + 40 mg Nano zinc oxide) achieved the best growth and feed conversion ratio (FCR). This experiment cleared that, nanoparticles Fe+Zn resulted in increasing the muscle content of Fe, while the liver and gills content of Fe was increased with bulk Fe+Zn. Also, bulk Fe+Zn led to an increase in the muscles, gills content of Zn in comparison with the liver content of Zn.