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67228

Use of microscopic and molecular techniques to assess removal of parasitic protozoa via conventional and compact drinking water treatment processes

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Last updated: 23 Dec 2024

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Abstract

The delivery of safe drinking water services depends on effective role played by stakeholders. From the parasitological point of view, the Egyptian standards for drinking water denied the presence of any type of living protozoan parasites in potable water produced for human use. In the present study, raw and treated water samples were separately collected from a conventional drinking water treatment plant (DWTP) and a compact unit. They were concentrated through nitrocellulose membrane filters (0.45µm pore size). The concentrate of each sample was examined with both light microscopy and PCR. Six genera of parasitic Protozoa (Giardia, Cryptosporidium, Entamoeba, Balantidium, Cyclospora and Isospora) and a group of microsporidial spores were detected in inlet water samples of the two examined treatment systems. Microscopically, the obtained data declared that 70.8% and 83.3% of inlet water from conventional DWTP and compact unit, respectively were contaminated with parasitic protozoa. The encountered parasitic protozoa in inlets of examined DWTPs were only microsporidial spores representing 8.3% and 16.7% of treated water from conventional DWTP and compact unit, respectively. Removal of protozoan parasites was higher in conventional DWTP (82.4%) than in compact unit (75%). Molecularly, the presence of protozoan parasites in the outlet of the compact unit was higher than in the outlet of the conventional DWTP. In general, molecular detection of protozoan parasites revealed a little bit lower or equal (in some instances) prevalence than that obtained by microscopy. In conclusion, most of inlet water samples from both conventional DWTP and compact unit were contaminated with protozoan parasites. Although conventional DWTP was more effective than compact unit for removing parasitic protozoa, l some of these parasites could be still detected in treated water and thus reach to consumers and cause health hazards.

DOI

10.21608/ejabf.2019.67228

Keywords

Enteric Protozoa, Parasites, Morphology, PCR, Water treatment, DWTP

Authors

First Name

Mahmoud

Last Name

A. Gad

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First Name

Fatma El-Zahraa

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R. Saleh

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First Name

Effat

Last Name

A. Morsy

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First Name

Mohamed

Last Name

A. Marouf

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First Name

Ahmad

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Z. Al-Herrawy

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Volume

23

Article Issue

5 (Special Issue)

Related Issue

9471

Issue Date

2019-12-01

Receive Date

2019-11-27

Publish Date

2019-12-27

Page Start

327

Page End

339

Print ISSN

1110-6131

Online ISSN

2536-9814

Link

https://ejabf.journals.ekb.eg/article_67228.html

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https://ejabf.journals.ekb.eg/service?article_code=67228

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22

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Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Use of microscopic and molecular techniques to assess removal of parasitic protozoa via conventional and compact drinking water treatment processes

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Article

Created At

22 Jan 2023