This study investigated the parameters affecting the performance of a 1000 mL dual-chamber MFC. A microbial fuel cell (MFC) reactor with two compartments has been constructed from cheap materials and used for electricity generation. The maximum power densities of 99.15 mW/m², 160.68 mW/m², 191 mW/m², and 204 mW/m² were obtained when aluminum electrode was used, the concentration of KCl in salt bridge was 6%, sodium acetate was used as a sole carbon source, and methylene blue used as a mediator; respectively.Changing the electrode materials, salt concentrations in the salt bridge, using different carbon sources and different mediators; had a great influence on electricity production from wastewaters using MFC. Microbial Fuel Cells (MFCs) offer the possibility of extracting electric current from a wide range of soluble or dissolved complex organic wastes and renewable biomass.