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70436

Isolation, partial purification and characterization of protease enzyme from the head of Nile tilapia fish (Oreochromis niloticus).

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Last updated: 23 Dec 2024

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Abstract

Fishery processing waste is as environmental problem. Fish waste, such as the head of the Nile tilapia fish (Oreochromis niloticus), is underutilized and has a low economic value, even though the waste can be re-processed.The purpose of this study was to isolate and purify protease enzymes from the head of Nile tilapia fish (O. niloticus) and to investigate their partial enzyme characteristics. Protease enzymes were isolated using various percentages of ammonium sulfate precipitation, which was followed by partial purification by dialysis. The total protein and specific activity of the enzyme was calculated at each stage of purification. The characteristics of the enzyme, namely optimum temperature, pH and Km and kcat were investigated. The result of investigation revealed that the 40% ammonium sulfate extraction showed the highest yield of the enzyme. Partial purification by dialysis can increase its specific activity 21 times from its crude extract form. The optimum temperature of the protease is 38°C, while the optimum pH is 7.5. The protease Km and kcat value of this protease were 1.887 mM and 39.768 s-1.The protease enzyme was optimally semi-purified using 40% ammonium sulfate precipitation. Judging from the result, this enzyme categorized as a neutral enzyme. Further analysis is needed to investigate its other characteristics.

DOI

10.21608/ejabf.2019.70436

Keywords

Fish waste, Nile tilapia, Oreochromis niloticus, Protease, Purification, Ammonium sulfate

Authors

First Name

Asep

Last Name

A. Prihanto

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Orcid

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First Name

Happy

Last Name

Nursyam

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Email

happy_nsy@ub.ac.id

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Orcid

0000-0002-6508-5493

First Name

Yoga

Last Name

D. Jatmiko

MiddleName

-

Affiliation

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Email

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City

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Orcid

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First Name

Royani

Last Name

L. Hayati

MiddleName

-

Affiliation

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Volume

23

Article Issue

3

Related Issue

5690

Issue Date

2019-07-01

Receive Date

2019-02-22

Publish Date

2019-07-01

Page Start

257

Page End

262

Print ISSN

1110-6131

Online ISSN

2536-9814

Article File

EJABF_Volume 23_Issue 3_Pages 257-262.pdf

PDF . 352.7kB

Link

https://ejabf.journals.ekb.eg/article_70436.html

Detail API

https://ejabf.journals.ekb.eg/service?article_code=70436

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Type

Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Isolation, partial purification and characterization of protease enzyme from the head of Nile tilapia fish (Oreochromis niloticus).

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Article

Created At

22 Jan 2023