Abstract The present study aimed to evaluate the antimicrobial activity ofChrysomya albiceps and Musca domestica maggots' extracts produced from different solvents against some bacterial and fungal strains, beside the role of these extracts as anti-HSV therapeutic agents. Results obtained evoked a variable activity against both Gram-positive and Gram-negative bacteria depending on tested species and the solvent used in extraction. The highest antibacterial activity was attained by petroleum ether extract 24h post treatment for both Gram-positive and Gram-negative bacteria either by Microbial Growth Inhibition method or by Minimum Inhibitory Concentration method followed by hexane, ethyl acetate and acetone extract. Gram-positive bacterial strains were more sensitive than Gram-negative bacterial strains. Regarding the antifungal activity, tested extracts showed variable antifungal activity. In addition, petroleum ether extracts of tested species exhibited a vital role as potential anti-HSV agent due to their promising antiviral activity. In general, tested extracts induced remarkable effects on both antimicrobial and antiviral activities.