The present study assesses reproductive biology of Yellowspotted Puffer Torquigener flavimaculosus, were collected seasonally from commercial catches at the Attaka fishing harbor in Suez from winter 2017 until autumn 2018. The sex ratio was found 1:1.08 for male and female, respectively. The fish length at first sexual maturity (L₅₀) was 8.2 cm for males and 9.5 cm for females. In addition, the allometric pattern of gonadal growth was studied to validate the use of the gonado-somatic index (GSI) in assessments of the reproductive cycle. The highest peak of GSI (10.5 ± 1.012%) and (4.3 ± 0.084%) for female and male were recorded in summer, respectively. Values for hepato-somatic index (HSI) is very high and strong inverse relationship with gonado-somatic index (GSI) we inferred that lipid reserves in the liver play an important role in gonad maturation and spawning. Somatic condition factor (Kr) also varied, albeit less so, throughout the year, suggesting that body fat and muscle play lesser roles in providing energy for reproduction. Seasonality of liver lipid content and different spawning seasons have important implications for designing sampling strategies using these fish, especially when  monitoring  lipophilic contaminants. According  to  the  present  results  of  seasonal  variations  in maturity  stages gonado-somatic  index  (GSI), hepato-somatic index (HSI) and  somatic condition factor (Kr) indicate that  the  reproductive  season  of Torquigener flavimaculosus  collected  from  Gulf  of  Suez represented in summer season.