A 120 day's feeding study was designed to study the effect of Lacto cel-con probiotic on the growth performance, hematology indices and body composition of Nile tilapia. Zea mays was substituted by sorghum in five formulated diets with inclusion levels of (0,25,50,75 and 100%). Fingerlings of mono-sex Nile tilapia with an initial weight of (1.94±0.13g) were distributed in1m³hapa fixed in earthen pond at stocking rate of 40 fish/replicate. The highest growth performance parameters in terms of (weight gain and specific growth rate) and nutrient utilization (feed conversion ratio and net protein utilization) were recorded by replacing 75 and 100% of Zea mays. On the other hand, blood indices include, Red blood Cell (RBCs) count, Lymphocyte, Hemoglobin concentration and Hematocrit value) displayed non-significant different among treatments. However, the values of White Blood Cell count (WBCs) were shown remarkable significance (P<0.05) with DS75 and DS100% diets. No significance differences were obtained in carcass composition of tilapia fed in different sorghum levels. Results showed that sorghum meal supplemented with (0.3% Lacto-cel con) can be entirely substituted up to75% of corn meal in fingerlings of mono-sex Nile tilapia diets without negative effects on growth performance, nutrient utilization, some blood parameters and body composition. It's conclude that (DS75) is a potential protein-rich with low cost feeding diet for mono-sex Nile tilapia (Oreochromis niloticus).