The Libyan coast plays an important role in terms of biodiversity and productivity of Mediterranean marine ecosystem. This study is designed to assess potential risks for human populations via fish intake. It enhances the information about   anthropogenic impacts in Tripoli port (Libya) to understand the distribution of pollutants encourage appropriate common policies to predict potential risk zones for stakeholders. The levels of Iron, Zinc, Copper, and Cadmium in livers, gills, muscles, skin, and bones of five Mediterranean Sea fish species in Tripoli Port (Libya) namely; Boops boops, Hemiramphus far, Sardinella aurita, Saurida undosquamis and Scomber japonicas were evaluated. The results showed that considerable difference in metal concentrations among fish organs. The highest concentrations of Cd, Cu, and Fe were measured in livers, while gills and skins had higher concentrations of Zn. The ranges of heavy metal concentrations in different organs of studied fish species were reported (Zn; 7.18 - 21.94, Cu; 1.89-7.03, Fe; 0.93-4.05, Cd; 0.19 to 0.97  µgg^-1wet wt.). Fortunately, the calculated weekly intakes of metals (EWIs) were much lower than provisional tolerable weekly intake (PTWI). So, human health risks resulting from consumption of studied fish species from Tripoli Port are inconsiderable, that fish muscles are not active tissues for metal accumulation, rendering them suitable for human consumption. In this study the hazard quotient (HQ) and the hazard index (HI) were <1, and so no potential health risk to the consumers. Principal component analyses specified that Cu and Cd were contributed from the same anthropogenic activity.