The present work is the second to the first one titled ''Bioaccumulation of Cadmium and Lead in the freshwater crayfish Procambarus clarkii from the River Nile, Egypt"(Shaaban et al., 2017). Crayfish Procambarus clarkii has been selected to assess some heavy metals accumulation pattern. This part aims to evaluate Iron, Zinc and Copper bio-accumulation in exoskeleton, muscles, hepatopancreas and ovary of this species as well as in water and sediment from the same sites for four seasons. Also, determination of risk assessment of Cadmium, Lead, Iron, Zinc and Copper. The results showed, Fe levels were higher than Zn and Cu concentrations in water, sediment and crayfish's organs in all sites. In water, the highest value of Fe was 1628.51μg/l recorded in El-Rayah El-Monoufy during summer, while Zn recorded highest value (60.6 µg/l) in spring at El-Rahawy. The highest value of Cu was 28.2µg/l at El-Warraq during summer. In sediment, Fe recorded the highest value (7005.5 μg/g) at El-Warraq during summer. Zn and Cu recorded the highest values (178.5 and 49.1 μg /g) at El-Rahawy during autumn and summer, respectively. In crayfish tissues, Fe was highest in the exoskeleton 126.13 μg/g at Helwan in summer while Cu and Zn were 37.17 and 43.74 μg/g respectively at El-Rahawy in autumn. On the other hand, the lowest concentrations of Fe, Zn, Cu were recorded in crayfish muscles (82.35, 37.85 and 17.14 μg /g respectively). For edible muscles, the results showed that, the values of Hazard index were within safe limits for Zn, Fe and Cu at all sites, while Cd values were detected only in El Rayah El Monoufy in safe limit. For Pb, HI values were unsafe at all sites. These results indicated, a larger amounts of crayfish will need to be eaten for health risk to be associated with Fe, Zn, Cu.