In hatcheries the induction of spawning in sea cucumbers has been typically carried out using different techniques and combinations of mixed induced spawning techniques, for the Holothuria atra for the first time combination of heat and cold chock were used to induce spawn samples collected from the Red Sea. Combination of rising water temperature by 10º C and then reduced by 10º C provoked animals to take the (Copra position) the reproductive position in sea cucumber. Gametes shedding have started about 60 min and 80 min after placing the animals back in normal sea temperature, and was completed almost simultaneously in the two sexes during 2 hours. Different other treatments were used including spirulina bath; exposure to water jet, draying and injection with Serotonin, however heat chock was the only successful technique. Larvae were fed on unicellular algae and Sargassum extract. Early juveniles were obtained on day 35. Survival rate was 4% to the juvenile st