Atotal of 300 swabs were taken from 100 Nile fish surfaces (3 swabs from each fish), in addition to 75 sewer swabs from
25 slaughterhouses (3 swabs from each sewer) at Gharbia and Kalyobia Governorates. All the swabs were subjected to
microbiological methods for isolation of Staphylococcus aureus, Salmonella spp. and Moulds and Yeasts. Staph. aureus could be isolated from 11% of the fish samples and 88% from the sewer samples. While Salmonella organisms were present in 8% of the sewer samples; they were absent in all samples. Aspergillus, Penicillium, Mucor, Candida, Rhodot could be isolated from both the sewer and fish samples. Moreover, isolates belonging to the genera Aspergillus and Candida were further identified to the species level. Aspergillus niger, A. fumigatus , A. flavus, Candida albicans, C. tropicalis and C. parapsillosis could be differentiated. The public health importance of the most isolated pathogenic organisms was given.