Monthly samples of Oreochromis niloticus from the River Nile at Beni Suef were collected during the period from January 2009 to December 2010. The Von Bertalanffy growth function parameters were: 𝐋∞ = 48.14 cm, K = 0.147 𝐲𝐫−𝟏, and 𝐭𝟎 = 0.2237 year. The total mortality coefficient (Z) was calculated as 0.9654 𝐲𝐫−𝟏, the natural computed mortality coefficient (M) was 0.4010 𝒚𝒓−𝟏 with fishing mortality (F) estimated as 0.5643 𝐲𝐫−𝟏 and exploitation ratio (E) calculated as 0.5819. This high exploitation ratio shows that the stock of Oreochromis niloticus from the River Nile at Beni Suef is overexploited.