The African catfish (Clarias gariepinus) fed on diets containing different lipid levels (5%, 10%, 15% and 20%) at a feeding rate of 3% of the live body weight. Fish were reared in glass aquaria at a stocking rate of 10 fish at each aquarium. Each dietary Iipid level was presented in triplicates. The trial lasted for 90 days from the start. Ten fish were distributed in 100-L glass aquaria. Fish fed daily for 90 days in triplicate form.Results revealed that fish growth parameters as a final weight, weight gain, and specific growth rate were generally increased by increasing dietary lipid levels up to 10%, after which the growth rate was markedly decreased. Differences in feed intake among different treatments varied significantly (PO.05). Feed conversion ratio (FCR) was affected by dietary levels of lipids. No significant changes in survrval rate were observed due to the variation in dietary lipid levels. The highest values of the erythrocyte count (RBCS), hemoglobin content (Hb) and packed cell volume (PCV) were obtained in fish group fed on the diet containing 15% and 20% Iipid. Furthermore, mean corpuscular value (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were changed in blood of fish fed on different levels of lipid. Glucose, total lipids, and total protein were significantly affected by increasing the lipid levels. Plasma total lipids and total protein were increased in fish with increasing lipid levels in the diets, Also, plasma AST and ALT activities increased significantly with increasing the level of lipids in fish diets, reaching the highest value in fish group fed diet with 20% lipids. The hepato-somatic index and gonado-somatic index of African catfish were significantly increased with increasing lipid level in fish diets. The present study demonstrated that the optimum dietary Iipid level was 10% for African catfish and there was no observed beneficial effect when fish was fed a diet containing 15% or 20% lipid levels.