The effect of seven types of bee-stored pollen (bee bread) from monofloral sources on ovarian development and egg-laying workers formation during queenless condition was studied in the apiary of Faculty of Agriculture, Assiut University.  Pollens play an important and main role in the ovaries development of bee workers.  All pollen types under experiment occurred well development in worker ovaries, in comparison with control (undevelopment).  Broad bean pollen gave the highest effect on ovarian development, whereas coriander pollen gave the lowest effect.  Workers fed on broad bean pollen gave the maximum value of ovarian development index (2.5), on the 15^th day, followed by canola pollen, the index was 2.55, on the 18^th day.  Whereas, all workers fed on each of caper, maize, clover, fennel and coriander pollen gave the maximum value of index (2.45, 2.35, 2.35, 2.05 and 2.05, respectively), on the 21^st day.  Appearance of egg-laying workers was sooner in workers fed on broad bean and canola pollen than others, (latency period = 9 days), followed by caper pollen (12 days).  While the latency period was 15 days for workers fed on maize, fennel and clover pollens.  The slowest appearance of egg-laying worker was observed on the 18^th day for workers fed on coriander pollen.