Bolti (Tilapia nilotica) Fish fingers were reared in water contained three levels of Al ions for two month. Technological parameters of a life fish as well as gross chemical composition of fish flesh were determined.  Soluble protein was fractionated by using Ultrathin-layer isoelectric focusing on polyacrylamide gel (150μm thickness). Al concentration was determined in the main three parts of fish (head, viscera and flesh). Results revealed that Al pollution of fish environment caused a reduction in fish weight and length as well as increase the weight of fish viscera. Consequently, fish stability as food declined. Significant (p ≤0.05) differences were noticed in chemical composition of Bolti fish due to the presence of Al ions in fish environment, where protein content of fish flesh decreased. while both mean ash and lipid content increased.Fish viscera had the significantly (p < /em>≤0.05) highest Al content followed by fish head and the lowest value was detected in fish flesh.The higher the Al concentration in water environment, the upper the Al content in fish parts. Isoelectric focusing of fish protein pattern was changed due to Al-pollution. Since, the lower the Al-pollution, the more color intensity of separated protein bands. In the meantime, some protein bands were totally disappeared between pH 7.3 to 8.3 at high Al concentration up 6 ppm in the reared water.