The present work aimed at improving pineapple (Ananas comosus (L.) Merrill) cv. Smooth Cayenne production throughout the tissue culture technique. Some factors affect this process were studied. The method of surface sterilization, the adventitious and axillary bud formation versus N⁶-Benzyladenine (BA) concentration, type of medium (liquid or solidified), rooting and acclimatization were evaluated. Explants have been surface sterilized with sodium hypochlorite (NaOCl) at 0.5 %, then with mercuric chloride (Hg Cl₂) at 0.1 % both for 5 minutes, with survival percentage of 95%. All explants were cultured onto MS basal medium supplemented with different concentrations of BA and 1.0 mg l^-1 Kinetin (Kin). All cultures were maintained in a growth room at a 16 h photoperiod (40 mmol.m^-2 s^-1), 27 ± 2°C. After eight weeks, number of initiated shoots was counted and the cultures were transferred for multiplication on the best treatment, 2.0 mg l^-1 BA and 1.0 mg l^-1 Kin. Therefore, using the liquid medium during multiplication stage speed up the growth of proliferated shoots compared to solidified medium. Utilizing 1.0 – 3.0 mg l^-1 α-Naphthaleneacetic acid (NAA) or Indole-3-butyric acid (IBA) enhanced the root formation of individual shoots. Finally, the well rooted plantlets, around 12 cm in length, were transplanted onto the Peat moss as a soil bed under the greenhouse. After 4 weeks, the survival percentage was 100 %. By this improved protocol, more than 10000 pineapple plants were produced from one initial plant in less than 9 months.