Two field experiments were carried out during the two successive seasons (2004 and 2005) in the Experimental Farm, Faculty of Agriculture, South Valley University, Qena, Egypt. The experiments studied the response of sesame cultivars Giza 32, Toushki 1 and Shandaweel 3 to foliar fertilization by micronutrients,biofertilizer (microbien) and Humix. 
Results showed that application of micronutrient + biofertilizer (microbien) + Humix significantly increased plant height, number of capsules /plant, 1000-seed weight, seed oil content seed yield /plant, and biological, straw, seed and oil yields /fad but decreased the height of first capsule, compared to the untreated control. Toushki 1 and Giza 32 cvs. surpassed Shandaweel 3 in seed yield and yield attributes, while seeds of Giza 32 cv. were richer in oil content over other cultivars. The highest yield and yield attributes were obtained when Toushki 1 or Giza 32 cvs. were treated with micronutrient + biofertilizer (microbien) + Humix.