A field experiment was carried out during 2003/2004 and 2004/2005 seasons in El- Wadi ElAssiuty farm, Faculty of Agriculture, Assiut University to study the response of canola plants fertilized by tacamolia organo-mineral fertilizer(TOMF) combination with mineral nitrogen fertilizers ( 1.0 ton TOMF +80kgN, 2.0 ton TOMF +70kgN , 3.0 ton TOMF + 60 kg N, 4.0 ton TOMF + 50kgN and 5.0 ton TOMF + 40 kgN/ha). Control canola plants were fertilized with 90 kg N/ha mineral nitrogen without adding TOMF and three hill spacing (10, 20 and 30 cm)in sandy calcareous soil under drip irrigation system. The obtained results indicated that TOMF at all tested rates significantly increased plant height, number of primary racemes/plant, number of siliques/plant, number of seeds/ siliqua, 1000-seed weight, seed yield / plant, seed oil percentage seed yield/ha as well as oil yield/ ha and water use efficiency compared with the control treatment with the superiority for the highest TOMF rate 5.0 ton +40kg N/ ha) which exhibited the upper most values of the previous traits. Seed oil percentage followed an opposite trend in the two seasons. Plants sowed at the widest space (30 cm) gained the highest values of the prementioned traits, except plant height in comparison with the other two hill spacing. The interaction between TOMF rates and hill spacing had a significant effect on the most tested traits in both seasons. The highest values of the previous traits were gained when canola plants were sowed at the widest space (30 cm) and fertilized with the highest TOMF rate (5.0ton TOMF +40kgN/ ha)