In the first hybrid of Carniolan bees, feeding preference on different types of bee bread from monofloral sources was studied in the apiary of Faculty of Agriculture, Assiut University. In the tests of feeding preference, newly emerged bee workers were given a choice among seven types of bee bread from maize, coriander, canola, caper, broad bean, Egyptian clover and fennel plants. Generally, in the period of 1-3 days, bees consumed the highest percentage of total pollens (51.36%) followed by the period of 4-6 days (36.25%) then the pollen consumed sharply decreased at the period of 7-9 days (9.22%). There were significant differences in pollen consumption of bee workers among all pollen types. The total consumption of pollen was 16.9, 14.4, 11.2, 9.7, 8.0, 5.4 and 0.6 mg/bee/18 days for bees fed on maize, coriander, canola, caper, broad bean, Egyptian clover and fennel pollens, respectively. The results indicated that honey bee workers exhibit a preference for pollen types more than the others. The present results suggested to divide the pollen consumed into three groups dependant on preference level of pollen feeding by bee workers. The first group (more preferred), pollen consumption was more than 20% from total pollen consumed, included maize (25.53%) and coriander pollen (21.75%). The second (considerably preferred), the pollen consumption ranged from 10-20%, included canola (16.92%), caper (14.65%) and broad bean pollen (12.08%). Whereas, the third (slightly preferred), the consumption percentage was less than 10%, included Egyptian clover (8.16%) and fennel pollen (0.91%). It can be summarized that the maize and coriander pollens were the most favourable and best attractant pollens than other tested pollens. While fennel pollen was less favourable and bad attractant.