The need for alternative water resources, coupled with increasingly stringent water quality discharge requirements, are the driving forces for developing wastewater reuse strategies in the world today, especially the arid and semiarid areas, i.e. Saudi Arabia. Reuse of fish pond effluent for crop production enables practitioners to manipulate the water cycle, thereby creating needed alternative water resources and reducing effluent discharge to the environment. The present study was designed to evaluate the effect of the reuse of fish pond effluent and its interaction with applied fertilizer on the production of sugar beet plants Beta vulgaris. It was clear that the irrigated sugar beet plants with fish pond effluent showed significant increases in root fresh weight yield, sucrose concentration and theoretical sugar yield (TSY) when compared with the irrigated plants with groundwater (control), especially at the lower and median N fertilizer levels (119 and 238 kg ha-1 )