The effect of the acarid mite, Mycetoglyphus qassimi (Acari: Acaridae) and chicken manure against the root-knot nematode, Meloidogyne javanica on tomato were evaluated under greenhouse conditions in Al Qassim, Saudi Arabia. The control of the root-knot nematode by using the acaried mite and chicken manure was compared with the effect of the nematicide Oxamyl (Vydate, 24%). Chicken manure highly accelerated the growth of both root and shoot systems of tomato in addition to suppressing root galls and egg masses of M. javanica. Chicken manure supported the acarid mite vitality and feeding capacity on M. javanica as compared with control treatments with nematode only. However, vydate application gave the highest effect in reducing both root galls and egg masses on root system and followed by mite treatments. The reduction percentages in numbers of nematode galls averaged 79.69% when tomato plants grown in chicken manure amended soil. This value was clearly decreased to 65,9% in soil that received mite individuals. Also, the effect of Vydate application was higher than mite introduction on the total number of egg masses of M. javanica especially in plants grown in chicken manure soil.  The effect of mite application on M. javanica was enhanced by combination with chicken manure amendment.