Beta
270069

CHARACTERIZATION OF PHYTASES FROM CEREAL GRAINS AS AFFECTED BY SOAKING AND GERMINATION PROCESSES AND SOME METALS IONS

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Food Science & Technology

Abstract

The properties of phytases of raw, soaked and germinated wheat, barley, sorghum and maize were studied. The optimal pH value was 5.0 for wheat, barley and sorghum phytases and 5.5 for maize germ phytase. The optimal temperature of wheat and barley phytases was 55oC while of sorghum and maize germ it was 50oC. Both optimal pH and temperature of phytases of different cereal grains did not affect by soaking or germination processes. Data revealed that maximal activity of phytase was found to be at 2.0 mM sodium phytate concentration from wheat, barley and sorghum and at 1.5 mM from maize germ. The calculated Michael's constant (Km) and their corresponding Vmax values of the extracted phytases from 96 h-germinated studied grains were varied. The enzyme activity was decreased with extending the incubation time more than 2 h for wheat, barley and maize germ phytase, and more than 1 h for sorghum phytase. Ca2+ ions addition caused less reduction of phytase activity of all studied cereals, whereas others metal ions had variable effect on reduction of enzyme activity. Wheat and sorghum showed high increase in their phytase activities after 120 h- germination. The same effect  was observed after 96 h-germination of barley and maize germ. At above germination times, phytase activity increased by 5.4-, 4.6-, 7.3- and 6.9-folds for wheat, barley, sorghum and maize germ, respectively compared with that before germination.

DOI

10.21608/ajas.2008.270069

Keywords

Phytase activity, cereal grains, Soaking, Germination, inactivation factors

Authors

First Name

A.

Last Name

Abdel - Gawad,

MiddleName

-

Affiliation

Dept of Food Science and Technology Faculty of Agriculture, Assiut University

Email

abdalla.saleh25@agr.aun.edu.eg

City

-

Orcid

-

First Name

B.

Last Name

Ramadan

MiddleName

-

Affiliation

Dept of Food Science and Technology Faculty of Agriculture, Assiut University

Email

bramadan1958@yahoo.com

City

Assiut

Orcid

-

First Name

Mohamed

Last Name

B. Omar

MiddleName

-

Affiliation

Dept of Food Science and Technology Faculty of Agriculture, Assiut University

Email

mohamed.nasr@agr.au.edu.eg

City

-

Orcid

-

First Name

Refat

Last Name

E.A. Oraby

MiddleName

-

Affiliation

Dept of Food Science and Technology Faculty of Agriculture, Assiut University

Email

-

City

-

Orcid

-

Volume

39

Article Issue

4

Related Issue

37667

Issue Date

2008-12-01

Receive Date

2008-08-01

Publish Date

2008-12-01

Page Start

17

Page End

35

Print ISSN

1110-0486

Online ISSN

2356-9840

Link

https://ajas.journals.ekb.eg/article_270069.html

Detail API

https://ajas.journals.ekb.eg/service?article_code=270069

Order

2

Type

Original Article

Type Code

62

Publication Type

Journal

Publication Title

Assiut Journal of Agricultural Sciences

Publication Link

https://ajas.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023