This study was carried out in apiary at Assiut region during November, 2007. The efficiency of repeated treatments using oxalic acid (3.2% concentration) with trickling and spraying methods to control varroa mites under brood decrease condition in honey bee colonies was studied.  After 24 hours of oxalic acid applications by trickling and spraying, the dead fallen mites were significantly higher in both methods (445.4 and 607.4 mites/colony, respectively) than control (21.2 mites/colony), resulting 20.0 and 27.7 efficiency index, whereas after 48 and 72 hours the results were similar to 24 hours but the levels of fallen mites were low.  The first application of oxalic acid by the two tested methods produced significantly more fallen mites (653.0 and 743.2 mites/colony, respectively), inducing 10.8 and 12.4 efficiency index.  Similarity, was in both of the second and the third applications by the two methods but were in lowest levels of fallen mites.  The cumulative efficiency of the three oxalic acid applications by trickling and spraying methods to control varroa mites in broodright colonies was 94.61 and 94.05% as compared with untreated control colonies.  The effectiveness of the trickling and spraying treatments was not significantly different. A non significant difference was observed in number of dead bees in the treated colonies with oxalic acid by both examined methods.  It can be recommended to control varroa mites of the beekeepers using the oxalic acid in concentration of 3.2%. Especially, using of trickling method because it was less consuming time and labor intensive yet had equivalent efficiency when compared with the spraying method.