The present study was carried out at the apiary of Faculty of Agriculture, Assiut University during active season of 2007.  The effect of different states of bee queens on the development of worker ovaries were studied.  These states of queens were: mated queens of 1-year-old, 1-month-old, supersedure, and virgin queens of 10, 7 and 3 day- old.  All queens states under experiment occurred highly significant inhibition in the ovaries development of bee workers, in compa-red to queenless control bees.  Bee workers headed with mated queens 1-year-old gave the minimum value of ovarian development index (1.02), inducing 95% ovarian inhibition as compared to queenless control workers.  Whereas the minimum percentage of inhibition was recorded by workers headed with virgin queen 3-days old (37.2%), resulted 1.27 ovarian index.  Stage I (slightly developed) of ovaries development of bee workers was appeared clearly on the 6^th day for all treatments, then increased to reach maximum value between 12^th and 18^th day.  The appearance of stage II (well developed) of ovaries development, which named egg-laying workers was recorded only in queenless control bees, on the 12^th day, that reached maximum percentage on the 18^th day (13.3%).