The present study was conducted through two successive seasons of (2007-2009) at Fac. Agric. Exptl. Farm. Assiut Univ. to survey Arthropods associated with three grapevine varieties (Muscat, Thompson seedless and Azmerly) and study the seasonal fluctuations of the most abundant and economically injurious pests: black vine thrips, Retithrips syriacus Mayet and European red mite, Panoychus ulmi Koch. Data of the survey study revealed the presence of 20 insect species belonging to 20 genera under 14 families of 8 orders. Moreover, mite species was represented by three species belonging to three genera under two families of two orders. Intensive and extensive observations indicated that the collected insect and mite species can be classified according to their economic importance as pests included 19 insect species and two mite species. Predators included one insect species and one mite species. In the first season (2007/2008) R. syriacus started with low levels in April, increased in May and the maximum number of individuals on leaves was recorded in June and July, for the three grapevine varieties. Then, the number of individuals decline rapidly during the next six months (Aug.-Jan.).