Field experiments were conducted, to evaluate the role of cultural practices for suppressing damage of the rodents and increasing the stalks and sugar yield in sugarcane field at Mallawi district, Minia Governorate, Middle Egypt, during the two successive seasons from July 2009 to February 2010 and July 2010 to February 2011. The results proved that the low susceptible cultivars to Nile grass rat, Arvicanthis niloticu infestation were Giza 47/88 (4.25, 5.90%); PH8013 (4.75, 5.20%) and Giza 88/68 (5.48, 6.43%), followed by the cultivars, Giza 75/368  (7.40, 9.0%) and G.T. 54/9 (7.0, 9.33%) while the  cultivar Giza 74/96 (10.39, 12.45%) received  the highest susceptible infestation based on number of percent infested internodes / stalk in plant and 1^st ratoon, respectively. The maximum reduction percentage of sucrose cane be caused by rodent infestation was noticed in Giza 74/96 variety (24.29 and 22.82), followed by Giza 75/368 (20.23 and13.79) and Giza 54/9 (19.31 and 15.92), while minimum reduction was recorded in Giza 88/68 (15.72 and 9.65); Ph8013 (14.71 and 7.02) and Giza 47/88 (12.11 and 7.72) in the main plant and 1^st ratoon cane, respectively.