The current indoor research trial was conducted in the mushroom research and production laboratory at the Department of Horticulture, the Faculty of Agriculture, Assiut University. Productivity of two oyster mushroom species (Pleurotus ostreatus and P. columbinus) was evaluated for ten different substrates. These included six sole and four blended substrate recipes. For the six sole substrates, three were target untraditionally used substrates studied against three commonly used substrates. The three target untraditional substrates were Cynodon dactylon grass weed (GW), African mahogany (Khaya senegalensis) tree leaves (TL) and Faba Bean (Vicia faba L.) straw (FBS). The three commonly used substrates were rice straw (RS), wheat straw (WS) and sugarcane bagasse (SCB). The four blended substrate recipes were prepared from equal weight of FBS and SCB with each ofGW, TL, RS and WS.  Data were recorded for days lapsed to visible pinheads formation (VPF), fruiting bodies yield (FBY), number of fruiting bodies per culture (NF), average fruiting body weight (FW) and biological efficiency (BE).