The aim of this work is to study the effect of Paecilomyces, Bacillus and Trichoderma as biocontrol against M. javanica and treatment times of biological control application on pepper under greenhouse conditions. A total of 75 plastic pots were divided into seven experimental groups. The first group was kept without infection as healthy control. Two weeks later, the pots of the other groups were inoculated with roughly 5000 eggs and j2 / pot. The second group, which acted as an infected control, was kept without treatments. The third to sixth groups were treated with bioagents (Paecilomyces (P.), Bacillus (B.), Trichoderma (T.) and Paecilomyces + Bacillus+ Trichoderma (P.B.T.), respectively. The 7th group received Vydate (nematocid). The 3rd to 6th groups were divided into three subgroups which received the biological treatments at three phases (Before- With- After) infection. These four biological controls treatments induced significant decrease in disease parameter compared with control. The highest reduction% in galls, egg masses and number of J2/250 cm3 of soil was obtained with Vydate application followed by those obtained with P.B.T when applicated before-, with- and after-infection then Paecilomyces when applicated before- and with-infection than the other treatments. All of the tested biological controls caused significant increases in the growth parameter of pepper (GPP). The highest in GPP was obtained with biological control application before- and with-infection.