Finding of new effective anti-cancer agents has become a top goal around the world. Thus, the objective of this study was to investigate the cytotoxic activities of guar seeds methanolic extract (GSME), guar seeds oil (GSO), guar gum (GG) and guar saponin (GSP) from seeds against huh-7 cancer cell line. For 72 hours, cells were exposed to 0.01, 0.1, 1.0, 10, 100 µg/ml of various guar seeds extracts (GSME, GSO, GG, and GSP), then the SRB assay was performed three times in triplicate to determine cell viability %, cell growth inhibition % and half-maximal inhibitory concentration (IC50). In addition, phase contrast inverted microscopy was used to examine the morphological changes in the cells. The huh-7 cells were shown to be cytotoxic to all guar seed extracts. Among the various examined preparations, guar seed saponin (GSP) was shown to be the most cytotoxic substance at a concentration of 100 µg/ml towards huh-7 cells. As a result, the guar seed tested extracts could potentially be used in the creation of anticancer drugs.