In contrast to yeast and animal cells, plants contain two genes which is encoding to nucleolin proteins. A comprehensive protein sequence analysis revealed that the two RNA recognition motifs (RRM) domains are highly conserved and suggesting that the mechanisms and functions related to RNA contained RRMs are highly conserved in both plant nucleolin proteins sequences. Whereas Ribonucleoprotein 1 (RNP1) and Ribonucleoprotein 2 (RNP2) that are highly conserved sequences in RRMs from yeast and animal nucleolin, also my study confirmed that RNP1 and RNP2 sequences are conserved in plants. Nucleolin could use the same manner in the different species of plants and animals to achieve its function. Study of evolutionary relationships among nine different plant species revealed that monocots and dicots form two separated monophyletic group meaning that they composed of a collection of organisms, share a common evolutionary history and including the most recent common ancestor of all those organisms.