In order, to assess the effects of foliar spry time by different micronutrients nanoparticles on sunflower cultivar, Sakha-53, a field experiment was organized in a randomized complete block design using strip plot arrangement with three replications during 2019 and 2020 seasons at Agronomy Department Farm, Agriculture Faculty, Assiut University, Egypt. Three different foliar spraying times of different micronutrients nanoparticles were allocated horizontally while eight treatments of spraying with different Micronutrient nanoparticles (2 gm/L) were arranged vertically. The results showed that foliar spraying twice after 30 and 50 days from sowing was significantly increased plant height, stem diameter and head diameter in both seasons. In addition, the highest mean values of seed yield (t fed.-1) and oil yield (kg fed.-1) were recorded when foliar spraying was done threes after 30,50 and 70 days from sowing. Foliar application of Iron, Manganese, and Zinc nanoparticles was superior all other treatments in this respect and registered the highest mean values of all studied yield attributes, seed yield, and oil yield in both seasons. The interaction between spraying time numbers and micronutrients nanoparticles significantly affected all sunflower yield attributes in both growing seasons. The highest mean values of seed yield and oil yield were obtained from the interaction between foliar spraying three times × spraying with Iron, Manganese and Zinc. It was concluded that foliar spray threes time using Iron, Manganese and Zinc nanoparticles could be used as a suitable method for improving seed yield and its attributes of sunflower cultivar, Sakha-53.