Five genotypes of garlic (Allium sativum L.), namely Balady (G1), Chinese (G2), Sids-40 (G3), Egaseed-1 (G4) and Egaseed-2 (G5) were used for assessing tissue culture response, genetic variability and polymorphism amongst which and to identify molecular markers associated with tissue culture response using ISSR and SRAP techniques. Significant differences were found among the five genotypes, the concentration of growth regulators as well as the interaction between them for all measured tissue culture traits. The Euclidean-distance dendrogram using tissue culture data separated the five garlic genotypes into two clusters; the first cluster comprised the highly responsive genotypes (G1, G2 and G3) while the less responsive genotypes (G4 and G5) grouped together in the second cluster.