A field trail was conducted at The Experimental Farm, Fac. of Agric., Al-Azahar Univ., Assiut, located 375 km south of Cairo, Egypt (27°  12^- 16.67⁼ N latitude and 3° 09^- 36.86⁼ E longitude) during the growth seasons of summer 2016 and winter 2016/2017 to assess the effect of tillage and nitrogen fertilization practices on soil organic matter partitioning into the functional compartments with different dynamics. The study included three plowing manner (No plowing, NP, Minimum plowing, MP and Traditional plowing, TP) and two nitrogen sources (Urea 46.5% N as a fast nitrogen fertilizer and ureaform 40% N as a slow nitrogen fertilizer).