During 2015 and 2016 seasons, Thompson Seedless grapevines were fertilized with organic fertilizer, compost, humic acid and bio fertilizer (Ascophyliumnodosum) algae. These fertilizers were used as a partial replacement for inorganic N fertilizer improving yield, lowering pollution and enhancing the efficiency of exportation to foreign markets. The obtained results indicated that application of N as 50% inorganic N plus50% organic N and algae 5ml / vine was the best management system for ensuring the best yield, number of clusters /vine and cluster weight, leaf mineral content and improving the chemical characteristics of berries as total soluble solids (TSS), total acidity (TA) and Tss /acid ratio and decreasing nitrate and nitrite content in the berries.