This study was carried out during 2015 and 2016 seasons to examine the effect of spraying amino acids enriched with N,P,K,Mg,Zn,Fe,Mn and B on overcoming irregular berries colouration problem and improving both yield and quality of the berries in grapevines cv. Flame seedless grown under Minia Governorate dimatic conditions. Spraying was done three times. Treating the vines three times with various nutrients besides amino acids at 0.1% was very effective in enhancing growth, vine nutritional status, berries colouration, yield and quality of the berries over the check treatment. Treating the vines with all nutrients (N,P,K,Mg,Zn,Fe,Mn and B) plus amino acids was superior than using nutrients alone in this respect. Using amino acids was essential for enhancing the efficiency of using nutrients. For promoting berries colouration, yield and quality of the berries in Flame seedless grapevines grown under Minia Governorate dimatic condition, it is necessary to use a mixture of NPKMgZnFeMnB and amino acids at 0.1%.