The experiment was conducted during the two successive seasons of 2015 and 2016 on Ruby Seedless grapevines grown in the Experimental Orchard, Faculty of Agriculture, Assiut University. This investigation was carried out to study the effect of spraying yeast, algae and fish oil on vegetative growth, nutrient status and fruiting. The experiment was set up in a randomized complete block design with three replicates, one vine per each. The obtained results could be summarized as follows: -  Pruning wood weight, leaf area and its nutritional status significantly increased with spraying yeast, algae and fish oil compared to unsprayed ones. Spraying yeast combined with algae plus fish oil at half concentration of single spraying gave the maximum values of these aspects. -  Spraying yeast combined with algae and fish oil gave the highest cluster number, heaviest clusters and yield/vine compared to the other spraying treatments and unsprayed ones. No significant differences were observed among double spraying form or triple ones. -  All yeast, algae or fish oil treatments significantly improved the berry quality in terms of increasing berry weight, total soluble solids %, reducing sugars % and skin berry anthocyanin and decreasing titratable acidity % compared to unsprayed ones. The present study concluded that carrying out sprays of mixture of 5 g/L yeast, 0.5 g/L algae or 0.5 ml/L fish oil improved the growth and nutritional status of vines, in addition get high yield with good cluster and berry attributes.