This investigation was conducted during the two successive seasons of 2015 and 2016 at the Experimental Orchard Faculty of Agriculture, Assiut University, Egypt, to compare the effects of GA₃ and lemongrass oil spraying on fruiting of Ruby Seedless grapevines. GA₃ spraying was applied twice, once at 5 or 7.5 ppm at full bloom and the second, 30 ppm when the berry size about 5-6 mm, whereas, lemongrass oil spraying was applied at 0.5, 1 or 1.5% at the same previous dates. The results obtained could be summarized under the following main points: lemongrass oil twice spraying significantly decreased the berry set percentage, cluster weight and yield/vine and consequently significantly decreased the cluster compactness coefficient compared to the unsprayed ones. On other hand, GA₃ twice spraying significantly decreased the berry set percentage and insignificantly decreased the cluster weight and yield/vine, as well as significantly decreased the cluster compactness compared to unsprayed ones. No significant differences in these traits due to lemongrass oil spray at 1 or 1.5% as well as GA₃ at 5 or 7.5 ppm. Lemongrass oil significantly improved the berry quality in terms of berry weight, total soluble solids and anthocyanin contents, whereas GA₃ significantly increased the berry weight and significantly decreased anthocyanin content. From this study, it is clear that lemongrass oil twice spray at 1% improve the grapevines production and overcome the adverse effects of GA₃ used at high concentration.