Pot experiments were conducted to study the effect of Arbuscular mycorrhizal fungi (AMF), Tricoderma harzianum and mix of them on suppression of fusarium wilt of three varieties of tomato (castle-rock, super marmande and peto 86). Disease severity, shoot and root Length, fresh, dry weights and the changes in amino acids, reducing sugars and phenolic compounds contents and phenylalanine ammonia lyase, ascorbate peroxidase, peroxidase and poly phenoloxidase activities were investigated. The treatments AMF, T. harzianum and AMF + T. harzianum gave significant reduction of disease severity and elevated phenolic acids, amino acids and reducing sugars contents and POD, PPO, APX and PAL activities in roots and leaves of tomato varieties.