Two field experiments were conducted in the Agricultural Research Station at Arab El-Awamer, Assiut Governorate, during the two growing seasons of 2014 and 2015 to investigate the response of two grain sorghum genotypes productivity to bio- and nitrogen fertilizer in newly reclaimed soil. The randomized complete block design using split-split-plot with four replications was employed, where the genotypes (Hybrid 305 and Dorado i.e. G₁ and G₂) were allocated in the main plots, while nitrogen fertilizer rates (60, 90 and 120 kg N/fed i.e. N₁, N₂ and N₃) were assigned in the sub-plots and bio-fertilizer (0, Microbin and Nitrobin i.e. B₀, B₁ and B₂) were fixed in the sub-sub-plots. The grains of sorghum genotypes were sown on 13^th and 17^th June in the first and second seasons, respectively. The plot area was 12 m² including 4 ridges of 60 cm apart with 4 m length. The results could be summarized as the following: -  Hybrid 305 genotype surpassed the Dorado variety and gained the highest mean values of the plant height, panicle length, panicle weight and grain yield/fed, while the Dorado variety gave the highest mean values of panicle width and 1000 grain weight in both seasons. -  The all studied traits increased by increasing N fertilizer rates and the maximum values were achieved by the highest N fertilizer rate (120 kg N/fed.) in both seasons. -  The Microbin bio-fertilizer recorded the highest mean values for the all studied traits in both seasons, but Nitrobin bio-fertilizer recorded the heaviest 1000 grain in the 1^st season only. -  The G₁xN₃ (Hybrid 305 x 120 kg N/fed.) interaction treatments gave the maximum values for panicle weight in both seasons and grain yield/fed. in the 2^nd season, as well as G₁xN₂ (Hybrid 305 x 90 kg N/fed.) gave the maximum values for grain yield/fed. in the 1^st season. Moreover, the maximum grain yield/fed. (18.89 and 14.62 ard.) were obtained by G1xB1 (Hybrid 305 x Microbin) interaction treatments in the 1^st and 2^nd seasons, respectively. -  In general, the first order interaction; N₁xB₀ (60 kg N/fed. x without bio-fertilizer) interaction treatment gave the lowest mean values for the all studied traits in both seasons. -  The highest mean values for panicle weight and grain yield/fed. were recorded by G₁xN₃xB₀ (Hybrid 305 x 120 kg N/fed. x without bio-fertilizer) and G₁xN₂xB₁ (Hybrid 305 x 90 kg N/fed. x Microbin) interaction treatments in the 1^st season, respectively.