This study aims to evaluate the effect of water stress on growth, nutrients uptake and yield of maize grown under drip irrigation. For this purpose field experiment in a randomized complete block design (RCBD) was carried out at the Agricultural Experimental Station farm of the Faculty of Agriculture, Assiut University, Egypt during the during the summer seasons of 2014 and 2015. Maize plants were irrigated by 100 or 75% of water requirements (I₁₀₀ and I₇₅). The obtained results of this study show that the irrigation of maize by I₁₀₀ significantly (P<0.05) increased the plant growth. Uptake of N, P, and K by maize irrigated by I₁₀₀ increased by 11, 7 and 16 % in the first season and by 13, 11 and 15% in the second season compared to that irrigated by I₇₅.Increasing the irrigation level to 100% caused a 20 and 6% increase in the straw yield in the first and second season, respectively, also it caused a 20% increase in the biological yield in the first season compared to I₇₅. The grain yield of maize irrigated by I₇₅ was higher by 5 and 10% in the first and second season, respectively, compared to I₁₀₀.  Water use efficiency (WUE) was higher by 41and 56% in the first and second season, respectively, in the case of I₇₅ compared to I₁₀₀. The data of the current study indicated that water stress caused a slightly reduction in the straw and biological yield of maize, on the other hand it caused a slightly increase in the grain yield. From this study it recommended maize irrigated by 75% of water requirements was higher of grain yield than that received 100% of water requirements.